16th International HLA and Immunogenetics Workshop

22 Mar 2012

16th International HLA and Immunogenetics Workshop : Liverpool : 28th May - 3rd
June 2012
Global Distribution of Extended HLA Haplotypes
Medhat Askar, Marcelo Fernandez-Vina


Objectives:
This workshop component aims at characterization and studying the distributions of extended HLA
haplotypes (A, C, B, MICA, DRB1, DRB3/4/5, DQA1, DQB1, DPA1, and DPB1) among different ethnic
populations. These haplotypes will be identified through family studies conducted historically in the context
of related donor searches (mainly HSCT but also solid organs when available) in participating laboratories.
This study is open to laboratories from all over the world including different ethnicities that are less
represented in HSC donor registries.
Methods:
Index subjects carrying haplotypes identified by family studies in families with known ethnicity will be
further typed to high resolution and untyped loci (DQB1, DQA1, DRB3/4/5, DPB1, DPA1, MICA):
Typing can be performed at the participating labs or at the Cleveland Clinic at no cost
Identified haplotypes that reach a specific threshold (e.g. top 10th percentile, appear in 3 independent
families, etc) will be tested for prevalence in unrelated donors tested in participating labs and
individuals who appear to have no mismatches to these haplotypes will be (DQB1, DQA1, DRB3/4/5,
DPB1, DPA1, MICA)
Phases & Timeline
This project has four phases (Laboratories may elect to participate in one or more of the project phases):
Laboratories can submit informative cases by contacting the coordinators of the project and by
providing (June –Nov 2011):
1.
a. HLA typing of all tested family members of informative cases
b. Ethnic origin of these cases.
c. Records of any subjects typed in the same lab and appear to have this haplotypes in their
genotype along with their ethnic origin.
Workshop testing 2. of genomic DNA for HLA (Dec 2011 -Feb 2012).
a. Index subjects carrying haplotypes identified by family studies in families with known ethnicity
will be further typed to high resolution and untyped loci (DQB1, DQA1, DRB3/4/5, DPB1,
DPA1, MICA): Typing can be performed at the participating labs or at the Cleveland Clinic at no
cost
b. Identified haplotypes that reach a specific threshold (e.g. top 10th percentile, appear in 3
independent families, etc) will be tested for prevalence in unrelated donors tested in participatinglabs and individual appear to have no mismatches to these haplotypes will be (DQB1, DQA1,
DRB3/4/5, DPB1, DPA1, MICA)
c. Submit results which will be incorporated into the project database.
3. Data analysis (March-May 2012):
a. The project database will be made available to participants who wish to analyze the data.
b. Appropriate statistical methods will be applied to calculate haplotypes frequencies in different
ethnic populations
4. The group will prepare a workshop component report for publication.
Contacts:
Please let us know if you would like to participate in the workshop. We will provide you with further
information as the project proceeds.
Medhat Askar,
Allogen Laboratories,
Cleveland Clinic
Email: AskarM [at] ccf [dot] org
Marcelo Fernandez-Vina,
Histocompatibility, Immunogenetics & Disease Profiling Laboratory,
Stanford University
Email: marcelof [at] stanford [dot] edu

Last modified on 22 Mar 2012


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